BROCHURES / DOCUMENTATION
APPLICATION NOTES
SCIENTIFIC PUBLICATIONS
You are researching: University of Toronto
Cell Type
Tissue and Organ Biofabrication
Skin Tissue Engineering
Drug Delivery
Biological Molecules
Solid Dosage Drugs
Stem Cells
Personalised Pharmaceuticals
Inducend Pluripotent Stem Cells (IPSCs)
Drug Discovery
Cancer Cell Lines
All Groups
- Printing Technology
- Biomaterial
- Ceramics
- Metals
- Bioinks
- Fibronectin
- Xanthan Gum
- Paeoniflorin
- Methacrylated Silk Fibroin
- Heparin
- Fibrinogen
- (2-Hydroxypropyl)methacrylamide (HPMA)
- Carrageenan
- Chitosan
- Glycerol
- Poly(glycidol)
- Agarose
- methacrylated chondroitin sulfate (CSMA)
- Silk Fibroin
- Methacrylated hyaluronic acid (HAMA)
- Gellan Gum
- Alginate
- Gelatin-Methacryloyl (GelMA)
- Cellulose
- Hyaluronic Acid
- Polyethylene glycol (PEG) based
- Collagen
- Gelatin
- Novogel
- Peptide gel
- α-Bioink
- Elastin
- Matrigel
- Methacrylated Chitosan
- Pectin
- Pyrogallol
- Fibrin
- Methacrylated Collagen (CollMA)
- Glucosamine
- Non-cellularized gels/pastes
- 2-hydroxyethyl) methacrylate (HEMA)
- Paraffin
- Polyphenylene Oxide
- Acrylamide
- SEBS
- Ionic Liquids
- Jeffamine
- Mineral Oil
- Salecan
- Zein
- poly(octanediol-co-maleic anhydride-co-citrate) (POMaC)
- Poly(itaconate-co-citrate-cooctanediol) (PICO)
- Polyvinylpyrrolidone (PVP)
- Salt-based
- Acrylates
- 2-hydroxyethyl-methacrylate (HEMA)
- Magnetorheological fluid (MR fluid – MRF)
- Poly(vinyl alcohol) (PVA)
- PEDOT
- Polyethylene
- Silicone
- Pluronic – Poloxamer
- Carbopol
- Epoxy
- poly (ethylene-co -vinyl acetate) (PEVA)
- Phenylacetylene
- Poly(N-isopropylacrylamide) (PNIPAAm)
- Poly(Oxazoline)
- Poly(trimethylene carbonate)
- Polyisobutylene
- Konjac Gum
- Gelatin-Sucrose Matrix
- Chlorella Microalgae
- Poly(Vinyl Formal)
- Thermoplastics
- Micro/nano-particles
- Biological Molecules
- Decellularized Extracellular Matrix (dECM)
- Solid Dosage Drugs
- Review Paper
- Application
- Tissue Models – Drug Discovery
- BioSensors
- Personalised Pharmaceuticals
- In Vitro Models
- Bioelectronics
- Industrial
- Robotics
- Medical Devices
- Electronics – Robotics – Industrial
- Biomaterial Processing
- Tissue and Organ Biofabrication
- Liver tissue Engineering
- Muscle Tissue Engineering
- Nerve – Neural Tissue Engineering
- Meniscus Tissue Engineering
- Heart – Cardiac Patches Tissue Engineering
- Adipose Tissue Engineering
- Trachea Tissue Engineering
- Ocular Tissue Engineering
- Intervertebral Disc (IVD) Tissue Engineering
- Vascularization
- Skin Tissue Engineering
- Drug Delivery
- Cartilage Tissue Engineering
- Bone Tissue Engineering
- Drug Discovery
- Institution
- Myiongji University
- Hong Kong University
- Veterans Administration Medical Center
- University of Applied Sciences Northwestern Switzerland
- University of Michigan, Biointerfaces Institute
- Sree Chitra Tirunal Institute
- Kaohsiung Medical University
- Baylor College of Medicine
- L'Oreal
- University of Bordeaux
- KU Leuven
- Abu Dhabi University
- University of Sheffield
- DTU – Technical University of Denmark
- Hefei University
- Rice University
- University of Barcelona
- INM – Leibniz Institute for New Materials
- University of Nantes
- Institute for Bioengineering of Catalonia (IBEC)
- University of Amsterdam
- Bayreuth University
- Ghent University
- National University of Singapore
- Adolphe Merkle Institute Fribourg
- Zurich University of Applied Sciences (ZHAW)
- Hallym University
- University of Wurzburg
- AO Research Institute (ARI)
- Chalmers University of Technology
- ETH Zurich
- Nanyang Technological University
- Utrecht Medical Center (UMC)
- University of Manchester
- University of Nottingham
- Trinity College
- National Institutes of Health (NIH)
- Rizzoli Orthopaedic Institute
- University of Bucharest
- Innotere
- Nanjing Medical University
- Ningbo Institute of Materials Technology and Engineering (NIMTE)
- Queen Mary University
- Royal Free Hospital
- SINTEF
- University of Central Florida
- University of Freiburg
- Halle-Wittenberg University
- CIC biomaGUNE
- Chiao Tung University
- University of Geneva
- Novartis
- Karlsruhe institute of technology
- Shanghai University
- Technical University of Dresden
- University of Michigan – School of Dentistry
- University of Tel Aviv
- Aschaffenburg University
- Univerity of Hong Kong
- Chinese Academy of Sciences
- Helmholtz Institute for Pharmaceutical Research Saarland
- Brown University
- Innsbruck University
- National Yang Ming Chiao Tung University
- Tiangong University
- Harbin Institute of Technology
- Montreal University
- Anhui Polytechnic
- Jiao Tong University
- University of Toronto
- Politecnico di Torino
- Biomaterials & Bioinks
- Bioprinting Technologies
- Bioprinting Applications
- Cell Type
- Organoids
- Meniscus Cells
- Skeletal Muscle-Derived Cells (SkMDCs)
- Hepatocytes
- Monocytes
- Neutrophils
- Macrophages
- Corneal Stromal Cells
- Mesothelial cells
- Adipocytes
- Synoviocytes
- Human Trabecular Meshwork Cells
- Epithelial
- Human Umbilical Vein Endothelial Cells (HUVECs)
- Spheroids
- Keratinocytes
- Neurons
- Endothelial
- CardioMyocites
- Osteoblasts
- Articular cartilage progenitor cells (ACPCs)
- Cancer Cell Lines
- Chondrocytes
- Fibroblasts
- Myoblasts
- Melanocytes
- Retinal
- Embrionic Kidney (HEK)
- β cells
- Pericytes
- Bacteria
- Tenocytes
- Stem Cells
AUTHOR
Year
2023
Journal/Proceedings
Biosensors and Bioelectronics
Reftype
Groups
AbstractEngineered neural tissues serve as models for studying neurological conditions and drug screening. Besides observing the cellular physiological properties, in situ monitoring of neurochemical concentrations with cellular spatial resolution in such neural tissues can provide additional valuable insights in models of disease and drug efficacy. In this work, we demonstrate the first three-dimensional (3D) tissue cultures with embedded optical dopamine (DA) sensors. We developed an alginate/Pluronic F127 based bio-ink for human dopaminergic brain tissue printing with tetrapodal-shaped-ZnO microparticles (t-ZnO) additive as the DA sensor. DA quenches the autofluorescence of t-ZnO in physiological environments, and the reduction of the fluorescence intensity serves as an indicator of the DA concentration. The neurons that were 3D printed with the t-ZnO showed good viability, and extensive 3D neural networks were formed within one week after printing. The t-ZnO could sense DA in the 3D printed neural network with a detection limit of 0.137 μM. The results are a first step toward integrating tissue engineering with intensiometric biosensing for advanced artificial tissue/organ monitoring.
AUTHOR
Title
Flexible 3D printed microwires and 3D microelectrodes for heart-on-a-chip engineering
[Abstract]
Year
2023
Journal/Proceedings
Biofabrication
Reftype
DOI/URL
DOI
Groups
AbstractWe developed a heart-on-a-chip platform that integrates highly flexible, vertical, 3D micropillar electrodes for electrophysiological recording and elastic microwires for the tissue’s contractile force assessment. The high aspect ratio microelectrodes were 3D-printed into the device using a conductive polymer, poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) (PEDOT:PSS). A pair of flexible, quantum dots/thermoplastic elastomer nanocomposite microwires were 3D printed to anchor the tissue and enable continuous contractile force assessment. The 3D microelectrodes and flexible microwires enabled unobstructed human iPSC-based cardiac tissue formation and contraction, suspended above the device surface, under both spontaneous beating and upon pacing with a separate set of integrated carbon electrodes. Recording of extracellular field potentials using the PEDOT:PSS micropillars was demonstrated with and without epinephrine as a model drug, non-invasively, along with in situ monitoring of tissue contractile properties and calcium transients. Uniquely, the platform provides integrated profiling of electrical and contractile tissue properties, which is critical for proper evaluation of complex, mechanically and electrically active tissues, such as the heart muscle under both physiological and pathological conditions.
AUTHOR
Title
High Throughput Omnidirectional Printing of Tubular Microstructures from Elastomeric Polymers
[Abstract]
Year
2022
Journal/Proceedings
Advanced Healthcare Materials
Reftype
DOI/URL
DOI
Groups
AbstractAbstract Bioelastomers have been extensively used in biomedical applications due to their desirable mechanical strength, tunable properties, and chemical versatility; however, 3D printing bioelastomers into microscale structures has proven elusive. Herein, a high throughput omnidirectional printing approach via coaxial extrusion is described that fabricated perfusable elastomeric microtubes of unprecedently small inner diameter (350-550 μm) and wall thickness (40-60 μm). The versatility of this approach was shown through the printing of two different polymeric elastomers, followed by photocrosslinking and removal of the fugitive inner phase. Designed experiments were used to tune the dimensions and stiffness of the microtubes to match that of native ex vivo rat vasculature. This approach afforded the fabrication of multiple biomimetic shapes resembling cochlea and kidney glomerulus and afforded facile, high-throughput generation of perfusable structures that can be seeded with endothelial cells for biomedical applications. Post-printing laser micromachining was performed to generate numerous micro-sized holes (5-20 μm) in the tube wall to tune microstructure permeability. Importantly, for organ-on-a-chip applications, the described approach took only 3.6 minutes to print microtubes (without microholes) over an entire 96-well plate device, in contrast to comparable hole-free structures that take between 1.5 to 6.5 days to fabricate using a manual 3D stamping approach. This article is protected by copyright. All rights reserved