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You are researching: Adipose Tissue Engineering
Cell Type
Tissue and Organ Biofabrication
Skin Tissue Engineering
Drug Delivery
Biological Molecules
Solid Dosage Drugs
Stem Cells
Personalised Pharmaceuticals
Inducend Pluripotent Stem Cells (IPSCs)
Drug Discovery
Cancer Cell Lines
All Groups
- Application
- Tissue Models – Drug Discovery
- Tissue and Organ Biofabrication
- Cartilage Tissue Engineering
- Bone Tissue Engineering
- Drug Delivery
- Skin Tissue Engineering
- Vascularization
- Nerve – Neural Tissue Engineering
- Meniscus Tissue Engineering
- Heart – Cardiac Patches Tissue Engineering
- Adipose Tissue Engineering
- Trachea Tissue Engineering
- Ocular Tissue Engineering
- Intervertebral Disc (IVD) Tissue Engineering
- Biomaterial Processing
- Drug Discovery
- Electronics – Robotics – Industrial
- BioSensors
- Personalised Pharmaceuticals
- Bioprinting Technologies
- Biomaterials & Bioinks
- Cell Type
- Organoids
- Meniscus Cells
- Skeletal Muscle-Derived Cells (SkMDCs)
- Macrophages
- Corneal Stromal Cells
- Stem Cells
- Chondrocytes
- Fibroblasts
- Myoblasts
- Cancer Cell Lines
- Articular cartilage progenitor cells (ACPCs)
- Osteoblasts
- Epithelial
- Human Umbilical Vein Endothelial Cells (HUVECs)
- Spheroids
- Keratinocytes
- Neurons
- Endothelial
- CardioMyocites
- Melanocytes
- Retinal
- Embrionic Kidney (HEK)
- β cells
- Pericytes
- Bacteria
- Tenocytes
- Bioprinting Applications
- Institution
- University of Barcelona
- Rice University
- Hefei University
- Abu Dhabi University
- University of Sheffield
- DTU – Technical University of Denmark
- INM – Leibniz Institute for New Materials
- Innsbruck University
- Montreal University
- Harbin Institute of Technology
- ETH Zurich
- Nanyang Technological University
- Utrecht Medical Center (UMC)
- University of Manchester
- University of Nottingham
- Trinity College
- Chalmers University of Technology
- AO Research Institute (ARI)
- University of Wurzburg
- Institute for Bioengineering of Catalonia (IBEC)
- University of Amsterdam
- Bayreuth University
- Ghent University
- National University of Singapore
- Adolphe Merkle Institute Fribourg
- Zurich University of Applied Sciences (ZHAW)
- Hallym University
- National Institutes of Health (NIH)
- Rizzoli Orthopaedic Institute
- University of Bucharest
- University of Geneva
- Novartis
- Karlsruhe institute of technology
- Shanghai University
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- University of Michigan – School of Dentistry
- University of Tel Aviv
- Aschaffenburg University
- Chiao Tung University
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- Halle-Wittenberg University
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- Nanjing Medical University
- Ningbo Institute of Materials Technology and Engineering (NIMTE)
- Queen Mary University
- Royal Free Hospital
- SINTEF
- University of Central Florida
- University of Freiburg
- Univerity of Hong Kong
- University of Nantes
- Myiongji University
- University of Applied Sciences Northwestern Switzerland
- University of Michigan, Biointerfaces Institute
- Sree Chitra Tirunal Institute
- Kaohsiung Medical University
- Baylor College of Medicine
- L'Oreal
- University of Bordeaux
- KU Leuven
- Veterans Administration Medical Center
- Hong Kong University
- Review Paper
- Printing Technology
- Biomaterial
- Thermoplastics
- Bioinks
- Xanthan Gum
- Paeoniflorin
- Alginate
- Gelatin-Methacryloyl (GelMA)
- Cellulose
- Hyaluronic Acid
- Polyethylene glycol (PEG) based
- Collagen
- Gelatin
- Gellan Gum
- Methacrylated hyaluronic acid (HAMA)
- Silk Fibroin
- Fibrinogen
- (2-Hydroxypropyl)methacrylamide (HPMA)
- Carrageenan
- Chitosan
- Glycerol
- Poly(glycidol)
- Agarose
- methacrylated chondroitin sulfate (CSMA)
- Novogel
- Peptide gel
- α-Bioink
- Elastin
- Matrigel
- Methacrylated Chitosan
- Pectin
- Pyrogallol
- Fibrin
- Methacrylated Collagen (CollMA)
- Glucosamine
- Non-cellularized gels/pastes
- Jeffamine
- Mineral Oil
- Pluronic – Poloxamer
- Silicone
- Polyvinylpyrrolidone (PVP)
- Salt-based
- Acrylates
- 2-hydroxyethyl-methacrylate (HEMA)
- Magnetorheological fluid (MR fluid – MRF)
- Poly(vinyl alcohol) (PVA)
- PEDOT
- Polyethylene
- Carbopol
- Epoxy
- poly (ethylene-co -vinyl acetate) (PEVA)
- Poly(N-isopropylacrylamide) (PNIPAAm)
- Poly(Oxazoline)
- Poly(trimethylene carbonate)
- Polyisobutylene
- Konjac Gum
- Gelatin-Sucrose Matrix
- Chlorella Microalgae
- Poly(Vinyl Formal)
- Phenylacetylene
- 2-hydroxyethyl) methacrylate (HEMA)
- Paraffin
- Polyphenylene Oxide
- Micro/nano-particles
- Biological Molecules
- Decellularized Extracellular Matrix (dECM)
- Solid Dosage Drugs
- Ceramics
- Metals
AUTHOR
Title
High-throughput fabrication of vascularized adipose microtissues for 3D bioprinting
[Abstract]
Year
2020
Journal/Proceedings
Journal of Tissue Engineering and Regenerative Medicine
Reftype
DOI/URL
DOI
Groups
AbstractAbstract For patients with soft tissue defects, repair with autologous in vitro engineered adipose tissue could be a promising alternative to current surgical therapies. A volume-persistent engineered adipose tissue construct under in vivo conditions can only be achieved by early vascularization after transplantation. The combination of 3D bioprinting technology with self-assembling microvascularized units as building blocks can potentially answer the need for a microvascular network. In the present study, co-culture spheroids combining adipose-derived stem cells (ASC) and human umbilical vein endothelial cells (HUVEC) were created with an ideal geometry for bioprinting. When applying the favourable seeding technique and condition, compact viable spheroids were obtained, demonstrating high adipogenic differentiation and capillary-like network formation after 7 and 14 days of culture, as shown by live/dead analysis, immunohistochemistry and RT-qPCR. Moreover, we were able to successfully 3D bioprint the encapsulated spheroids, resulting in compact viable spheroids presenting capillary-like structures, lipid droplets and spheroid outgrowth after 14 days of culture. This is the first study that generates viable high-throughput (pre-)vascularized adipose microtissues as building blocks for bioprinting applications using a novel ASC/HUVEC co-culture spheroid model, which enables both adipogenic differentiation while simultaneously supporting the formation of prevascular-like structures within engineered tissues in vitro.
AUTHOR
Title
Bioprinting predifferentiated adipose-derived mesenchymal stem cell spheroids with methacrylated gelatin ink for adipose tissue engineering
[Abstract]
Year
2020
Journal/Proceedings
Journal of Materials Science: Materials in Medicine
Reftype
Colle2020
DOI/URL
DOI
Groups
AbstractThe increasing number of mastectomies results in a greater demand for breast reconstruction characterized by simplicity and a low complication profile. Reconstructive surgeons are investigating tissue engineering (TE) strategies to overcome the current surgical drawbacks. 3D bioprinting is the rising technique for the fabrication of large tissue constructs which provides a potential solution for unmet clinical needs in breast reconstruction building on decades of experience in autologous fat grafting, adipose-derived mesenchymal stem cell (ASC) biology and TE. A scaffold was bioprinted using encapsulated ASC spheroids in methacrylated gelatin ink (GelMA). Uniform ASC spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system. ASC spheroids in adipogenic differentiation medium (ADM) were evaluated through live/dead staining, histology (HE, Oil Red O), TEM and RT-qPCR. Viable spheroids were obtained for up to 14 days post-printing and showed multilocular microvacuoles and successful differentiation toward mature adipocytes shown by gene expression analysis. Moreover, spheroids were able to assemble at random in GelMA, creating a macrotissue. Combining the advantage of microtissues to self-assemble and the controlled organization by bioprinting technologies, these ASC spheroids can be useful as building blocks for the engineering of soft tissue implants.