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You are researching: Ocular Tissue Engineering
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AUTHOR Song, Min Jae and Quinn, Russ and Nguyen, Eric and Hampton, Christopher and Sharma, Ruchi and Park, Tea Soon and Koster, Céline and Voss, Ty and Tristan, Carlos and Weber, Claire and Singh, Anju and Dejene, Roba and Bose, Devika and Chen, Yu-Chi and Derr, Paige and Derr, Kristy and Michael, Sam and Barone, Francesca and Chen, Guibin and Boehm, Manfred and Maminishkis, Arvydas and Singec, Ilyas and Ferrer, Marc and Bharti, Kapil
Title Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration [Abstract]
Year 2022
Journal/Proceedings Nature Methods
Reftype Song2022
DOI/URL DOI
Abstract
Age-related macular degeneration (AMD), a leading cause of blindness, initiates in the outer-blood-retina-barrier (oBRB) formed by the retinal pigment epithelium (RPE), Bruch’s membrane, and choriocapillaris. The mechanisms of AMD initiation and progression remain poorly understood owing to the lack of physiologically relevant human oBRB models. To this end, we engineered a native-like three-dimensional (3D) oBRB tissue (3D-oBRB) by bioprinting endothelial cells, pericytes, and fibroblasts on the basal side of a biodegradable scaffold and establishing an RPE monolayer on top. In this 3D-oBRB model, a fully-polarized RPE monolayer provides barrier resistance, induces choriocapillaris fenestration, and supports the formation of Bruch’s-membrane-like structure by inducing changes in gene expression in cells of the choroid. Complement activation in the 3D-oBRB triggers dry AMD phenotypes (including subRPE lipid-rich deposits called drusen and choriocapillaris degeneration), and HIF-α stabilization or STAT3 overactivation induce choriocapillaris neovascularization and type-I wet AMD phenotype. The 3D-oBRB provides a physiologically relevant model to studying RPE-choriocapillaris interactions under healthy and diseased conditions.
AUTHOR Włodarczyk-Biegun, Małgorzata K. and Villiou, Maria and Koch, Marcus and Muth, Christina and Wang, Peixi and Ott, Jenna and del Campo, Aranzazu
Title Melt Electrowriting of Graded Porous Scaffolds to Mimic the Matrix Structure of the Human Trabecular Meshwork [Abstract]
Year 2022
Journal/Proceedings ACS Biomaterials Science & Engineering
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DOI/URL DOI
Abstract
The permeability of the human trabecular meshwork (HTM) regulates eye pressure via a porosity gradient across its thickness modulated by stacked layers of matrix fibrils and cells. Changes in HTM porosity are associated with increases in intraocular pressure and the progress of diseases such as glaucoma. Engineered HTMs could help to understand the structure–function relation in natural tissues and lead to new regenerative solutions. Here, melt electrowriting (MEW) is explored as a biofabrication technique to produce fibrillar, porous scaffolds that mimic the multilayer, gradient structure of native HTM. Poly(caprolactone) constructs with a height of 125–500 μm and fiber diameters of 10–12 μm are printed. Scaffolds with a tensile modulus between 5.6 and 13 MPa and a static compression modulus in the range of 6–360 kPa are obtained by varying the scaffold design, that is, the density and orientation of the fibers and number of stacked layers. Primary HTM cells attach to the scaffolds, proliferate, and form a confluent layer within 8–14 days, depending on the scaffold design. High cell viability and cell morphology close to that in the native tissue are observed. The present work demonstrates the utility of MEW for reconstructing complex morphological features of natural tissues.
AUTHOR Wang, Ruiqi and Deng, Shuai and Wu, Yuping and Wei, Haiying and Jing, Guangping and Zhang, Bosong and Liu, Fengzhen and Tian, Hui and Chen, Xiongbiao and Tian, Weiming
Title Remodelling 3D printed GelMA-HA corneal scaffolds by cornea stromal cells [Abstract]
Year 2022
Journal/Proceedings Colloid and Interface Science Communications
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DOI/URL URL DOI
Abstract
Engineering scaffolds with a structure mimicking that of native cornea allows for addressing the severe donor shortage for the corneal blindness treatment, which, however, remains challenging. In the light that corneal stromal (CS) cells can play a key role in corneal stroma formation, in this study we incorporated CS cells into three-dimensional (3D) scaffolds printed from hyaluronic acid-modified gelatin-methacrylate (GelMA-HA) scaffolds and characterized the scaffolds in terms of remodeled extracellular matrix (ECM) in vitro. Our results illustrated that the modification of GelMA by HA allowed for 3D printing of corneal scaffolds and further improved the characteristics of primary rabbit-derived corneal stromal cells for remodelling scaffolds. After 60 days, we decellularized the remodeled corneal scaffolds and examined their optical properties; and our results demonstrated that the 3D printed corneal scaffolds provided CS cells with cues that guided them toward the directional and spatial organization and facilitated the ECM remodelling.
AUTHOR Fenton, Owen S. and Paolini, Marion and Andresen, Jason L. and Müller, Florence J. and Langer, Robert
Title Outlooks on Three-Dimensional Printing for Ocular Biomaterials Research [Abstract]
Year 2019
Journal/Proceedings Journal of Ocular Pharmacology and Therapeutics
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DOI/URL DOI
Abstract
Abstract Given its potential for high-resolution, customizable, and waste-free fabrication of medical devices and in vitro biological models, 3-dimensional (3D) bioprinting has broad utility within the biomaterials field. Indeed, 3D bioprinting has to date been successfully used for the development of drug delivery systems, the recapitulation of hard biological tissues, and the fabrication of cellularized organ and tissue-mimics, among other applications. In this study, we highlight convergent efforts within engineering, cell biology, soft matter, and chemistry in an overview of the 3D bioprinting field, and we then conclude our work with outlooks toward the application of 3D bioprinting for ocular research in vitro and in vivo.