BROCHURES / DOCUMENTATION
APPLICATION NOTES
SCIENTIFIC PUBLICATIONS
You are researching: Montreal University
Cell Type
Tissue and Organ Biofabrication
Skin Tissue Engineering
Drug Delivery
Biological Molecules
Solid Dosage Drugs
Stem Cells
Personalised Pharmaceuticals
Inducend Pluripotent Stem Cells (IPSCs)
Drug Discovery
Cancer Cell Lines
All Groups
- Biomaterial
- Non-cellularized gels/pastes
- Mineral Oil
- poly(octanediol-co-maleic anhydride-co-citrate) (POMaC)
- Poly(N-isopropylacrylamide) (PNIPAAm)
- Poly(Oxazoline)
- Zein
- Acrylamide
- Poly(trimethylene carbonate)
- 2-hydroxyethyl) methacrylate (HEMA)
- Pluronic – Poloxamer
- Polyisobutylene
- Paraffin
- Ionic Liquids
- Silicone
- Konjac Gum
- Polyphenylene Oxide
- Polyvinylpyrrolidone (PVP)
- Gelatin-Sucrose Matrix
- Salt-based
- Chlorella Microalgae
- Acrylates
- Poly(Vinyl Formal)
- 2-hydroxyethyl-methacrylate (HEMA)
- Phenylacetylene
- Salecan
- Magnetorheological fluid (MR fluid – MRF)
- Poly(vinyl alcohol) (PVA)
- PEDOT
- Jeffamine
- SEBS
- Polyethylene
- Carbopol
- Epoxy
- Poly(itaconate-co-citrate-cooctanediol) (PICO)
- poly (ethylene-co -vinyl acetate) (PEVA)
- Micro/nano-particles
- Biological Molecules
- Bioinks
- (2-Hydroxypropyl)methacrylamide (HPMA)
- Methacrylated Collagen (CollMA)
- Carrageenan
- Glucosamine
- Chitosan
- Glycerol
- Poly(glycidol)
- Alginate
- Agarose
- Gelatin-Methacryloyl (GelMA)
- methacrylated chondroitin sulfate (CSMA)
- Cellulose
- Novogel
- Methacrylated Silk Fibroin
- Hyaluronic Acid
- Peptide gel
- Polyethylene glycol (PEG) based
- α-Bioink
- Heparin
- Collagen
- Elastin
- Gelatin
- Matrigel
- Gellan Gum
- Methacrylated Chitosan
- Methacrylated hyaluronic acid (HAMA)
- Pectin
- Silk Fibroin
- Pyrogallol
- Xanthan Gum
- Fibronectin
- Fibrinogen
- Fibrin
- Paeoniflorin
- Ceramics
- Decellularized Extracellular Matrix (dECM)
- Metals
- Solid Dosage Drugs
- Thermoplastics
- Non-cellularized gels/pastes
- Bioprinting Technologies
- Bioprinting Applications
- Cell Type
- Retinal
- Corneal Stromal Cells
- Chondrocytes
- Embrionic Kidney (HEK)
- Fibroblasts
- β cells
- Hepatocytes
- Myoblasts
- Pericytes
- Cancer Cell Lines
- Bacteria
- Articular cartilage progenitor cells (ACPCs)
- Tenocytes
- Monocytes
- Mesothelial cells
- Osteoblasts
- Neutrophils
- Adipocytes
- Epithelial
- Human Umbilical Vein Endothelial Cells (HUVECs)
- Organoids
- Synoviocytes
- Stem Cells
- Spheroids
- Meniscus Cells
- Keratinocytes
- Skeletal Muscle-Derived Cells (SkMDCs)
- Human Trabecular Meshwork Cells
- Neurons
- Macrophages
- Endothelial
- CardioMyocites
- Melanocytes
- Institution
- Helmholtz Institute for Pharmaceutical Research Saarland
- Chalmers University of Technology
- Karlsruhe institute of technology
- University of Freiburg
- University of Toronto
- Brown University
- AO Research Institute (ARI)
- Shanghai University
- Univerity of Hong Kong
- Montreal University
- University of Wurzburg
- Technical University of Dresden
- University of Nantes
- Harbin Institute of Technology
- Institute for Bioengineering of Catalonia (IBEC)
- University of Michigan – School of Dentistry
- Myiongji University
- Anhui Polytechnic
- University of Amsterdam
- University of Tel Aviv
- University of Applied Sciences Northwestern Switzerland
- Jiao Tong University
- Bayreuth University
- Aschaffenburg University
- University of Michigan, Biointerfaces Institute
- Abu Dhabi University
- Ghent University
- Chiao Tung University
- Sree Chitra Tirunal Institute
- University of Sheffield
- National University of Singapore
- CIC biomaGUNE
- Kaohsiung Medical University
- DTU – Technical University of Denmark
- National Yang Ming Chiao Tung University
- Adolphe Merkle Institute Fribourg
- Halle-Wittenberg University
- Baylor College of Medicine
- INM – Leibniz Institute for New Materials
- Tiangong University
- Zurich University of Applied Sciences (ZHAW)
- Innotere
- L'Oreal
- ETH Zurich
- Hallym University
- Nanjing Medical University
- University of Bordeaux
- Innsbruck University
- Politecnico di Torino
- Nanyang Technological University
- National Institutes of Health (NIH)
- Ningbo Institute of Materials Technology and Engineering (NIMTE)
- KU Leuven
- Utrecht Medical Center (UMC)
- Rizzoli Orthopaedic Institute
- Queen Mary University
- Veterans Administration Medical Center
- Chinese Academy of Sciences
- University of Manchester
- University of Bucharest
- Royal Free Hospital
- Hong Kong University
- University of Barcelona
- University of Nottingham
- University of Geneva
- SINTEF
- Rice University
- Trinity College
- Novartis
- University of Central Florida
- Hefei University
- Biomaterials & Bioinks
- Application
- Bioelectronics
- Industrial
- Biomaterial Processing
- Tissue Models – Drug Discovery
- In Vitro Models
- Robotics
- Drug Discovery
- Medical Devices
- Electronics – Robotics – Industrial
- Tissue and Organ Biofabrication
- Adipose Tissue Engineering
- Trachea Tissue Engineering
- Ocular Tissue Engineering
- Muscle Tissue Engineering
- Intervertebral Disc (IVD) Tissue Engineering
- Liver tissue Engineering
- Cartilage Tissue Engineering
- Bone Tissue Engineering
- Drug Delivery
- Skin Tissue Engineering
- Vascularization
- Nerve – Neural Tissue Engineering
- Meniscus Tissue Engineering
- Heart – Cardiac Patches Tissue Engineering
- BioSensors
- Personalised Pharmaceuticals
- Review Paper
- Printing Technology
AUTHOR
Year
2022
Journal/Proceedings
Bioprinting
Reftype
Groups
AbstractThermosensitive chitosan (CH)-based hydrogels prepared with a mix of sodium bicarbonate and β-glycerophosphate as gelling agents rapidly pass from a liquid at room temperature to a mechanically strong solid at body temperature without any crosslinker. They show excellent potential for tissue engineering applications and could be interesting candidates for bioprinting. Unfortunately, since gelation is not instantaneous, formulations compatible with cell encapsulation (chitosan concentrations around 2% or lower) lead to very poor resolution and fidelity due to filament spreading. Here, we investigate the FRESH bioprinting approach with a warm sacrificial support bath, to overcome these limitations and enhance their bioprintability. First, a support bath, made of Pluronic including sodium chloride salt as a rheology modifier agent, was designed to meet the specific physical state requirements (solid at 37 °C and liquid at room temperature) and rheological properties appropriate for bioprinting. This support bath presented yield stress of over 100 Pa, a shear thinning behavior, and fast self-healing during cyclic recovery tests. Three different chitosan hydrogels (CH2%w/v, CH3%w/v, and a mixture of CH and gelatin) were tested for their ability to form filament and 3D structures, with and without a support bath. Both the resolution and mechanical properties of the printed structure were drastically enhanced using the FRESH method, with an approximate four fold decrease of the filament diameter which is close to the needle diameter. The printed structures were easily harvested without altering their shape by cooling down the support bath, and do not swell when immersed in PBS. Live/dead assays confirmed that the viability of encapsulated mesenchymal stem cells was highest in CH2% and that the support bath-assisted bioprinting process did not adversely impact cell viability. This study demonstrates that using a warm FRESH-like approach drastically enhances the potential for bioprinting of the thermosensitive biodegradable chitosan hydrogels and opens up a wide range of applications for 3D models and tissue engineering.