BROCHURES / DOCUMENTATION
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SCIENTIFIC PUBLICATIONS
You are researching: Montreal University
Cancer Cell Lines
Cell Type
Tissue and Organ Biofabrication
Skin Tissue Engineering
Drug Delivery
Biological Molecules
Solid Dosage Drugs
Stem Cells
Personalised Pharmaceuticals
Inducend Pluripotent Stem Cells (IPSCs)
Drug Discovery
All Groups
- Printing Technology
- Biomaterial
- Ceramics
- Metals
- Bioinks
- Fibronectin
- Xanthan Gum
- Paeoniflorin
- Methacrylated Silk Fibroin
- Heparin
- Fibrinogen
- (2-Hydroxypropyl)methacrylamide (HPMA)
- Carrageenan
- Chitosan
- Glycerol
- Poly(glycidol)
- Agarose
- methacrylated chondroitin sulfate (CSMA)
- Silk Fibroin
- Methacrylated hyaluronic acid (HAMA)
- Gellan Gum
- Alginate
- Gelatin-Methacryloyl (GelMA)
- Cellulose
- Hyaluronic Acid
- Polyethylene glycol (PEG) based
- Collagen
- Gelatin
- Novogel
- Peptide gel
- α-Bioink
- Elastin
- Matrigel
- Methacrylated Chitosan
- Pectin
- Pyrogallol
- Fibrin
- Methacrylated Collagen (CollMA)
- Glucosamine
- Non-cellularized gels/pastes
- 2-hydroxyethyl) methacrylate (HEMA)
- Paraffin
- Polyphenylene Oxide
- Acrylamide
- SEBS
- Ionic Liquids
- Jeffamine
- Mineral Oil
- Salecan
- Zein
- poly(octanediol-co-maleic anhydride-co-citrate) (POMaC)
- Poly(itaconate-co-citrate-cooctanediol) (PICO)
- Polyvinylpyrrolidone (PVP)
- Salt-based
- Acrylates
- 2-hydroxyethyl-methacrylate (HEMA)
- Magnetorheological fluid (MR fluid – MRF)
- Poly(vinyl alcohol) (PVA)
- PEDOT
- Polyethylene
- Silicone
- Pluronic – Poloxamer
- Carbopol
- Epoxy
- poly (ethylene-co -vinyl acetate) (PEVA)
- Phenylacetylene
- Poly(N-isopropylacrylamide) (PNIPAAm)
- Poly(Oxazoline)
- Poly(trimethylene carbonate)
- Polyisobutylene
- Konjac Gum
- Gelatin-Sucrose Matrix
- Chlorella Microalgae
- Poly(Vinyl Formal)
- Thermoplastics
- Micro/nano-particles
- Biological Molecules
- Decellularized Extracellular Matrix (dECM)
- Solid Dosage Drugs
- Review Paper
- Application
- Tissue Models – Drug Discovery
- BioSensors
- Personalised Pharmaceuticals
- In Vitro Models
- Bioelectronics
- Industrial
- Robotics
- Medical Devices
- Electronics – Robotics – Industrial
- Biomaterial Processing
- Tissue and Organ Biofabrication
- Liver tissue Engineering
- Muscle Tissue Engineering
- Nerve – Neural Tissue Engineering
- Meniscus Tissue Engineering
- Heart – Cardiac Patches Tissue Engineering
- Adipose Tissue Engineering
- Trachea Tissue Engineering
- Ocular Tissue Engineering
- Intervertebral Disc (IVD) Tissue Engineering
- Vascularization
- Skin Tissue Engineering
- Drug Delivery
- Cartilage Tissue Engineering
- Bone Tissue Engineering
- Drug Discovery
- Institution
- Myiongji University
- Hong Kong University
- Veterans Administration Medical Center
- University of Applied Sciences Northwestern Switzerland
- University of Michigan, Biointerfaces Institute
- Sree Chitra Tirunal Institute
- Kaohsiung Medical University
- Baylor College of Medicine
- L'Oreal
- University of Bordeaux
- KU Leuven
- Abu Dhabi University
- University of Sheffield
- DTU – Technical University of Denmark
- Hefei University
- Rice University
- University of Barcelona
- INM – Leibniz Institute for New Materials
- University of Nantes
- Institute for Bioengineering of Catalonia (IBEC)
- University of Amsterdam
- Bayreuth University
- Ghent University
- National University of Singapore
- Adolphe Merkle Institute Fribourg
- Zurich University of Applied Sciences (ZHAW)
- Hallym University
- University of Wurzburg
- AO Research Institute (ARI)
- Chalmers University of Technology
- ETH Zurich
- Nanyang Technological University
- Utrecht Medical Center (UMC)
- University of Manchester
- University of Nottingham
- Trinity College
- National Institutes of Health (NIH)
- Rizzoli Orthopaedic Institute
- University of Bucharest
- Innotere
- Nanjing Medical University
- Ningbo Institute of Materials Technology and Engineering (NIMTE)
- Queen Mary University
- Royal Free Hospital
- SINTEF
- University of Central Florida
- University of Freiburg
- Halle-Wittenberg University
- CIC biomaGUNE
- Chiao Tung University
- University of Geneva
- Novartis
- Karlsruhe institute of technology
- Shanghai University
- Technical University of Dresden
- University of Michigan – School of Dentistry
- University of Tel Aviv
- Aschaffenburg University
- Univerity of Hong Kong
- Chinese Academy of Sciences
- Helmholtz Institute for Pharmaceutical Research Saarland
- Brown University
- Innsbruck University
- National Yang Ming Chiao Tung University
- Tiangong University
- Harbin Institute of Technology
- Montreal University
- Anhui Polytechnic
- Jiao Tong University
- University of Toronto
- Politecnico di Torino
- Biomaterials & Bioinks
- Bioprinting Technologies
- Bioprinting Applications
- Cell Type
- Organoids
- Meniscus Cells
- Skeletal Muscle-Derived Cells (SkMDCs)
- Hepatocytes
- Monocytes
- Neutrophils
- Macrophages
- Corneal Stromal Cells
- Mesothelial cells
- Adipocytes
- Synoviocytes
- Human Trabecular Meshwork Cells
- Epithelial
- Human Umbilical Vein Endothelial Cells (HUVECs)
- Spheroids
- Keratinocytes
- Neurons
- Endothelial
- CardioMyocites
- Osteoblasts
- Articular cartilage progenitor cells (ACPCs)
- Cancer Cell Lines
- Chondrocytes
- Fibroblasts
- Myoblasts
- Melanocytes
- Retinal
- Embrionic Kidney (HEK)
- β cells
- Pericytes
- Bacteria
- Tenocytes
- Stem Cells
AUTHOR
Year
2022
Journal/Proceedings
Bioprinting
Reftype
Groups
AbstractThermosensitive chitosan (CH)-based hydrogels prepared with a mix of sodium bicarbonate and β-glycerophosphate as gelling agents rapidly pass from a liquid at room temperature to a mechanically strong solid at body temperature without any crosslinker. They show excellent potential for tissue engineering applications and could be interesting candidates for bioprinting. Unfortunately, since gelation is not instantaneous, formulations compatible with cell encapsulation (chitosan concentrations around 2% or lower) lead to very poor resolution and fidelity due to filament spreading. Here, we investigate the FRESH bioprinting approach with a warm sacrificial support bath, to overcome these limitations and enhance their bioprintability. First, a support bath, made of Pluronic including sodium chloride salt as a rheology modifier agent, was designed to meet the specific physical state requirements (solid at 37 °C and liquid at room temperature) and rheological properties appropriate for bioprinting. This support bath presented yield stress of over 100 Pa, a shear thinning behavior, and fast self-healing during cyclic recovery tests. Three different chitosan hydrogels (CH2%w/v, CH3%w/v, and a mixture of CH and gelatin) were tested for their ability to form filament and 3D structures, with and without a support bath. Both the resolution and mechanical properties of the printed structure were drastically enhanced using the FRESH method, with an approximate four fold decrease of the filament diameter which is close to the needle diameter. The printed structures were easily harvested without altering their shape by cooling down the support bath, and do not swell when immersed in PBS. Live/dead assays confirmed that the viability of encapsulated mesenchymal stem cells was highest in CH2% and that the support bath-assisted bioprinting process did not adversely impact cell viability. This study demonstrates that using a warm FRESH-like approach drastically enhances the potential for bioprinting of the thermosensitive biodegradable chitosan hydrogels and opens up a wide range of applications for 3D models and tissue engineering.