BROCHURES / DOCUMENTATION
APPLICATION NOTES
SCIENTIFIC PUBLICATIONS
You are researching: β cells
Drug Delivery
Biological Molecules
Solid Dosage Drugs
Stem Cells
Personalised Pharmaceuticals
Inducend Pluripotent Stem Cells (IPSCs)
Drug Discovery
Cancer Cell Lines
Cell Type
Tissue and Organ Biofabrication
Skin Tissue Engineering
All Groups
- Bioprinting Applications
- Cell Type
- Osteoblasts
- Monocytes
- Mesothelial cells
- Epithelial
- Neutrophils
- Adipocytes
- Human Umbilical Vein Endothelial Cells (HUVECs)
- Organoids
- Stem Cells
- Spheroids
- Meniscus Cells
- Synoviocytes
- Keratinocytes
- Skeletal Muscle-Derived Cells (SkMDCs)
- Neurons
- Macrophages
- Human Trabecular Meshwork Cells
- Endothelial
- CardioMyocites
- Melanocytes
- Retinal
- Chondrocytes
- Embrionic Kidney (HEK)
- Corneal Stromal Cells
- Fibroblasts
- β cells
- Myoblasts
- Pericytes
- Cancer Cell Lines
- Bacteria
- Articular cartilage progenitor cells (ACPCs)
- Tenocytes
- Institution
- Ghent University
- Chiao Tung University
- Sree Chitra Tirunal Institute
- University of Sheffield
- National University of Singapore
- CIC biomaGUNE
- Kaohsiung Medical University
- DTU – Technical University of Denmark
- Adolphe Merkle Institute Fribourg
- Halle-Wittenberg University
- Baylor College of Medicine
- INM – Leibniz Institute for New Materials
- National Yang Ming Chiao Tung University
- Zurich University of Applied Sciences (ZHAW)
- Innotere
- L'Oreal
- Tiangong University
- ETH Zurich
- Hallym University
- Nanjing Medical University
- University of Bordeaux
- Innsbruck University
- Nanyang Technological University
- National Institutes of Health (NIH)
- Ningbo Institute of Materials Technology and Engineering (NIMTE)
- KU Leuven
- Politecnico di Torino
- Utrecht Medical Center (UMC)
- Rizzoli Orthopaedic Institute
- Queen Mary University
- Veterans Administration Medical Center
- University of Manchester
- University of Bucharest
- Royal Free Hospital
- Hong Kong University
- University of Barcelona
- Chinese Academy of Sciences
- University of Nottingham
- University of Geneva
- SINTEF
- Rice University
- Trinity College
- Novartis
- University of Central Florida
- Hefei University
- Chalmers University of Technology
- Karlsruhe institute of technology
- University of Freiburg
- AO Research Institute (ARI)
- Shanghai University
- Univerity of Hong Kong
- University of Toronto
- University of Wurzburg
- Technical University of Dresden
- University of Nantes
- Montreal University
- Institute for Bioengineering of Catalonia (IBEC)
- University of Michigan – School of Dentistry
- Myiongji University
- Harbin Institute of Technology
- University of Amsterdam
- University of Tel Aviv
- University of Applied Sciences Northwestern Switzerland
- Anhui Polytechnic
- Bayreuth University
- Aschaffenburg University
- University of Michigan, Biointerfaces Institute
- Abu Dhabi University
- Jiao Tong University
- Biomaterials & Bioinks
- Application
- Electronics – Robotics – Industrial
- Tissue and Organ Biofabrication
- Cartilage Tissue Engineering
- Bone Tissue Engineering
- Drug Delivery
- Skin Tissue Engineering
- Vascularization
- Nerve – Neural Tissue Engineering
- Meniscus Tissue Engineering
- Heart – Cardiac Patches Tissue Engineering
- Adipose Tissue Engineering
- Trachea Tissue Engineering
- Ocular Tissue Engineering
- Intervertebral Disc (IVD) Tissue Engineering
- Muscle Tissue Engineering
- BioSensors
- Personalised Pharmaceuticals
- Biomaterial Processing
- Tissue Models – Drug Discovery
- Drug Discovery
- Review Paper
- Printing Technology
- Biomaterial
- Decellularized Extracellular Matrix (dECM)
- Metals
- Solid Dosage Drugs
- Thermoplastics
- Non-cellularized gels/pastes
- Salt-based
- Chlorella Microalgae
- Acrylates
- Poly(Vinyl Formal)
- 2-hydroxyethyl-methacrylate (HEMA)
- Phenylacetylene
- Magnetorheological fluid (MR fluid – MRF)
- Salecan
- Poly(vinyl alcohol) (PVA)
- PEDOT
- Jeffamine
- Polyethylene
- SEBS
- Carbopol
- Epoxy
- poly (ethylene-co -vinyl acetate) (PEVA)
- Poly(itaconate-co-citrate-cooctanediol) (PICO)
- Poly(N-isopropylacrylamide) (PNIPAAm)
- Mineral Oil
- poly(octanediol-co-maleic anhydride-co-citrate) (POMaC)
- Poly(Oxazoline)
- Poly(trimethylene carbonate)
- 2-hydroxyethyl) methacrylate (HEMA)
- Zein
- Pluronic – Poloxamer
- Polyisobutylene
- Paraffin
- Silicone
- Konjac Gum
- Polyphenylene Oxide
- Ionic Liquids
- Polyvinylpyrrolidone (PVP)
- Gelatin-Sucrose Matrix
- Micro/nano-particles
- Biological Molecules
- Bioinks
- Gelatin-Methacryloyl (GelMA)
- methacrylated chondroitin sulfate (CSMA)
- Cellulose
- Novogel
- Hyaluronic Acid
- Peptide gel
- Methacrylated Silk Fibroin
- Polyethylene glycol (PEG) based
- α-Bioink
- Collagen
- Elastin
- Heparin
- Gelatin
- Matrigel
- Gellan Gum
- Methacrylated Chitosan
- Methacrylated hyaluronic acid (HAMA)
- Pectin
- Silk Fibroin
- Pyrogallol
- Xanthan Gum
- Fibrinogen
- Fibrin
- Paeoniflorin
- (2-Hydroxypropyl)methacrylamide (HPMA)
- Methacrylated Collagen (CollMA)
- Carrageenan
- Glucosamine
- Chitosan
- Glycerol
- Poly(glycidol)
- Alginate
- Agarose
- Ceramics
- Bioprinting Technologies
AUTHOR
Title
Collagen-Tannic Acid Spheroids for β-Cell Encapsulation Fabricated Using a 3D Bioprinter
[Abstract]
Year
2022
Journal/Proceedings
Advanced Materials Technologies
Reftype
DOI/URL
DOI
Groups
AbstractAbstract Type 1 Diabetes results from autoimmune response elicited against β-cell antigens. Nowadays, insulin injections remain the leading therapeutic option. However, injection treatment fails to emulate the highly dynamic insulin release that β-cells provide. 3D cell-laden microspheres have been proposed during the last years as a major platform for bioengineering insulin-secreting constructs for tissue graft implantation and a model for in vitro drug screening platforms. Current microsphere fabrication technologies have several drawbacks: the need for an oil phase containing surfactants, diameter inconsistency of the microspheres, and high time-consuming processes. These technologies have widely used alginate for its rapid gelation, high processability, and low cost. However, its low biocompatible properties do not provide effective cell attachment. This study proposes a high-throughput methodology using a 3D bioprinter that employs an ECM-like microenvironment for effective cell-laden microsphere production to overcome these limitations. Crosslinking the resulting microspheres with tannic acid prevents collagenase degradation and enhances spherical structural consistency while allowing the diffusion of nutrients and oxygen. The approach allows customization of microsphere diameter with extremely low variability. In conclusion, a novel bio-printing procedure is developed to fabricate large amounts of reproducible microspheres capable of secreting insulin in response to extracellular glucose stimuli.
AUTHOR
Year
2018
Journal/Proceedings
Bioprinting
Reftype
Groups
AbstractThe importance of 3D printing technologies increased significantly over the recent years. They are considered to have a huge impact in regenerative medicine and tissue engineering, since 3D bioprinting enables the production of cell-laden 3D scaffolds. Transition from academic research to pharmaceutical industry or clinical applications, however, is highly dependent on developing a robust and well-known process, while maintaining critical cell characteristics. Hence, a directed and systematic approach to 3D bioprinting process development is required, which also allows for the monitoring of these cell characteristics. This work presents the development of a flow cytometry-based analytical strategy as a tool for 3D bioprinting research. The development was based on a model process using a commercially available alginate-based bioink, the β-cell line INS-1E, and direct dispensing as 3D bioprinting method. We demonstrated that this set-up enabled viability and proliferation analysis. Additionally, use of an automated sampler facilitated high-throughput screenings. Finally, we showed that each process step, e.g. suspension of cells in bioink or 3D printing, cross-linking of the alginate scaffold after printing, has a crucial impact on INS-1E viability. This reflects the importance of process optimization in 3D bioprinting and the usefulness of the flow cytometry-based analytical strategy described here. The presented strategy has a great potential as a cell characterisation tool for 3D bioprinting and may contribute to a more directed process development.